In the current development of nanomedicine, the problem is not the lack of therapeutic substances but an efficient way of delivering them. On contact with body fluids, nanomaterials are immediately coated with proteins. This rapidly forming protein corona determines the physicochemical properties of nanoparticles. Moreover, the interaction with the cell membrane and the cell absorption mechanism is significantly controlled by the proteins adsorbed on the surface of the carrier. Therefore, the protein corona defines the biological identity of nanoparticles, influencing their cytotoxicity, biodistribution in the body and endocytosis to specific cells. The signature of the protein corona depending on the type of nanocarrier indicates its diversity and complexity and makes it difficult to predict its final action. By defining the role of individual proteins, it is possible to identify specific proteins responsible for the biological pathway of nanocarriers. So far, it has been assumed that predominantly quantitative proteins or proteins with dedicated biological functions have a significant impact on the achievement of the molecular target. Understanding which proteins found in the protein corona define the nanoparticle pathway is critical to the appropriate design of targeted nanocarriers.
Optimizing the properties of nanocarriers by using the protein corona for specific biomedical applications appears to be a promising tool in personalized medicine.
It should be emphasized that the use of nanomaterials in biomedical systems is currently relatively limited (the number of FDA-approved nanosystems is still less than 30). The presently observed difficulties in the development of nanomedicine are related to the response of the immune system resulting, inter alia, from the action of the protein corona. Therefore, there is a need for systematic research aimed at determining the specific role of the protein corona in biological systems. Advances in this field will allow the development of knowledge in the field of nanotoxicology, but also bring closer the compatibility of in vitro and in vivo models, and thus facilitate the achievement of the expected therapeutic effects at the stage of clinical trials.
ACHIEVEMENTS
Publications
- Szota M., Szwedowicz U., Rembiałowska N., Janicka-Klos A., Doveiko D., Chen Y., Kulbacka J., Jachimska B.* “Dendrimer Platforms for Targeted Doxorubicin Delivery—Physicochemical Properties in Context of Biological Responses” International Journal of Molecular Sciences 2024, 25(13), 7201.
- Adamczyk O., Szota M., Rakowski K., Prochownik M., Doveiko D., Chen Y., Jachimska B.* “Bovine Serum Albumin as a Platform for Designing Biologically Active Nanocarriers—Experimental and Computational Studies” International Journal of Molecular Sciences 2024, 25, 37.
- Tworek P., Rakowski K., Szota M., Lekka M., Jachimska B.*, “Changes in Secondary Structure and Properties of Bovine Serum Albumin as a Result of Interactions with Gold Surface” ChemPhysChem, 2024, 25(2):e202300505.
- Szota M. and Jachimska B.*, “Effect of Alkaline Conditions on Forming an Effective G4.0 PAMAM Complex with Doxorubicin” Pharmaceutics, 2023, 15(3), 875
- Szota M., Wolski P., Carucci C., Cesare F. M., Gurgul J., Panczyk T., Salis A., Jachimska B.*, “Effect of Ionization Degree of Poly(amidoamine) Dendrimer and 5-Fluorouracil on the Efficiency of Complex Formation—A Theoretical and Experimental Approach” International Journal of Molecular Sciences, 2023, 24, 819
Conferences
Oral presentations
- A. Kamińska, Ł. Luśtyk, J. Gurgul, B. Jachimska, “Comparison conformational stability of lysozyme adsorbed on the gold surface and artificial lipid membrane”, 7th International Symposium on Surface Imaging/Spectroscopy at the Solid/Liquid Interface, 2024
- B. Jachimska, A. Kamińska, K. Rakowski, Ł. Lustyk, P. Komorek “Effect of the Surface Charge Density on Changes in α-Synuclein Structure” ACS Spring 2024, American Chemical Society National Meeting section Division of Colloid and Surface Chemistry. 18.03.2024
- B. Jachimska, “Impact on Nanoparticles Bio-behavior: Protein Corona Formation”, University of Cagliari, Italy 2023
- B. Jachimska, “Structure and function of protein corona at the nanoparticles interface”, „Copernicus Dialogues” series titled -The Focus of Polish-Italian cooperation in the area of biomedicine, 27.10.2023, Roma, Italy
- A. Kamińska, J. Szechyńska, P. Komorek, B. Jachimska “The analysis of the influence of selected factors on changes in α-synuclein structure”, Book of Abstracts. 5st International Wroclaw Scientific Meeting, Wrocław, 19-21.10.2023, p. 26 [ISSN 2451-2680]
- K. Rakowski, P. Mulheran, K. Kubiak-Ossowska, B. Jachimska “Effect of Tau protein phosphorylation on the process of its adsorption on the neuronal membrane”, Book of Abstracts, 5th International Wroclaw Scientific Meeting, Wrocław, 2023, p.41 [ISSN 2451-2680]
- K. Rakowski, P. Mulheran, K. Kubiak-Ossowska, B. Jachimska “Badanie wpływu fosforylacji na mechanizm adsorpcji białka Tau na powierzchni modelowych błon komórek nerwowych”, Book of Abstracts, Nano(&)BioMateriały – od teorii do aplikacji 2023, Toruń, 2023, p.50 [ISBN 978-83-231-4215-7]
- B. Jachimska, P. Komorek, K. Rakowski, M. Szota “Conformational Stability of Bovine Serum Albumin as a Result of Interactions with Gold Surface” ACS Fall 2023, American Chemical Society National Meeting, San Francisco, 2023
- M. Szota, B. Jachimska ” G4.0 PAMAM dendrimer as an effective nanocarrier for anticancer drugs” ACS Fall 2023, American Chemical Society National Meeting section Division of Colloid and Surface Chemistry (Nanomaterials), San Francisco, 2023
- M. Szota, B. Jachimska „Dendrymer G4.0 PAMAM jako efektywny nanonośnik leków przeciwnowotworowych” Materiały Konferencyjne, V Interdyscyplinarna Konferencja Nano (&) BioMateriały – od teorii do aplikacji, Toruń, 2023, p. 49 [ISBN 978-83-231-4215-7]
- B. Jachimska, M. Szota, K. Rakowski, “Fibrinogen corona on the dendrimer nanoparticles” 4th ISFMS — Biochemistry, Molecular Biology and Druggability of Proteins, Florencja, Italy, 06-09.09.2022r.
- B. Jachimska, M. Szota, K. Rakowski, „Formation of fibrinogen corona on the dendrimer nanoparticles” section Colloid & Surface Chemistry Fall 2022, American Chemical Society National Meeting, 21– 25.08.2022r
- M. Szota, B. Jachimska, “Protein corona formation phenomenon around poly(amidoamine) dendrimers as potential nanocarriers for oncology applications”, section Polymeric Materials Science and Engineering (Innovations in drug delivery), Fall 2022, American Chemical Society National Meeting, 21 – 25.08.2022r
Poster presentations
- K. Rakowski, B. Jachimska, “Monitoring the interaction of α-synuclein with lipid membrane using molecular dynamics”, VI Interdisciplinary Conference Nano(&)BioMaterials – from theory to application, Toruń 2024
- K. Rakowski, P. Mulheran, K. Kubiak-Ossowska, B. Jachimska “Effect of Tau protein phosphorylation on the process of its adsorption on the neuronal membrane”, Book of Abstracts, ACS FALL 2023 – Harnessing the Power of Data, San Francisco, USA 2023
- B. Jachimska, M. Szota, O. Michel, J. Kulbacka „ Physicochemical properties and biological activity of dendrimer complex with fluorouracil” Book of abstract, Advances in Clinical and Experimental Medicine, 5th International Wroclaw Scientific Meeting, Wrocław, Poland, 2023, p. 67 [ISSN 2451-2680]
- B. Jachimska, M. Szota, U. Szwedowicz, N. Rembiałkowska, A. Janicka-Kłos, J. Kulbacka „Theranostic nanocarriers of doxorubicin based on dendrimer” Book of abstract, Advances in Clinical and Experimental Medicine, 5th International Wroclaw Scientific Meeting, Wrocław, Poland, 2023, p. 68 [ISSN 2451-2680]
- B. Jachimska, P. Komorek, K. Rakowski, M. Szota “Conformational Stability of Bovine Serum Albumin as a Result of Interactions with Gold Surface” American Chemical Society National Meeting, San Francisco, 2023
- K. Rakowski, M. Szota, B. Jachimska, “Albumin as a platform for designing biologically active carriers” 4th ISFMS—Biochemistry, Molecular Biology and Druggability of Proteins, Florencja, Italy, 06-09.09.2022r
- M. Szota, B. Jachimska “Functionalization of PAMAM dendrimers by the Protein Corona” 4th ISFMS-Biochemistry, Molecular Biology and Druggability of Proteins, Florencja, Italy, 06-09.09.2022
Awards
- Distinction of the presentation entitled “Effect of Tau protein phosphorylation on the process of its adsorption on the neuronal membrane”, presented by K. Rakowski at 5th International Wroclaw Scientific Meeting, Wrocław, Polska 2023, p.41 [ISSN 2451-2680]
- Distinction of the presentation entitled “Conformational Stability of Bovine Serum Albumin as a Result of Interactions with Gold Surface” presented by B. Jachimska at ACS Fall 2023, American Chemical Society National Meeting, San Francisco, 2023
- Distinction of the presentation entitled „ Dendrimer G4.0PAMAM dendrimer as an effective nanocarrier for anticancer drugs” presented by Magdalena Szota at 5th Interdisciplinary Conference Nano(&)BioMaterials – from theory to application, ISBN 978-83-231-4215-7]
- Young Investigator Training Programme 2019 Award for the oral presentation entitled “Poly(amidoamine) dendrimers as nanocarriers for anticancer drugs and their interactions with plasma proteins” presented by Magdalena Szota at Interfaces International Conference From New Materials to Life Science – Structure, Interactions, Dynamics and Activity
Defense of bachelor’s theses within the project
- Olga Adamczyk, “Biopolymers as controlled drug carriers”, Faculty of Materials Science and Physics, Cracow University of Technology, 2023
- Magdalena Prochownik “Polymer nanoparticles as controlled drug carriers” Faculty of Materials Science and Physics, Cracow University of Technology, 2023
Defense of master’s theses carried out within the project
- Klaudia Zaręba, “Aspects of protein conformational stability in the context of neurodegenerative diseases”, Cracow University of Technology 2023, the master thesis received the IKiFP PAN award
- Callum Meldrum, “Physiochemical Properties 4th generation of Poly(amidoamine) Dendrimers complexes with doxorubicin”, Department of Chemical & Process Engineering, University of Strathclyde UK 2023
Defense of doctoral theses carried out within the project
- Magdalena Agnieszka Szota, “Poly(amidoamine) dendrimers as functional delivery systems for anticancer drugs – physicochemical characterization and biological activity”, 2024
