Protein adsorption and functional hybrid carriers

Agnieszka Kamińska

Agnieszka Kamińska, PhD Eng

12 6395 212

Education

2014 – 2019 

PhD in Physics, Jagiellonian University in Krakow, The Faculty of Physics, Astronomy and Applied Computer Science, Department of Medical Physics. Research area: Biophysics, Biomaterials, Biomedicine 

Thesis: „Molecular characteristics of platelet and urinary extracellular vesicles and their potential application in nanomedicine”

 

Completed:  28 June 2012 

Master of Science diploma of second cycle Full-time Course of Study programme in the field of Biomedical Engineering with major in Biomaterials and tissue engineering, University of Science and Technology, Kraków, Multidisciplinary School of Engineering in Biomedicine

 

Completed:  27 Jan 2011 

Engineer diploma of first cycle Full-time Course of Study programme in the field of Biomedical Engineering, University of Science and Technology, Kraków, Multidisciplinary School of Engineering in Biomedicine 

Projects

  1. Principal Investigator in the Lider IX project financed by the National Centre for Research and Development, Nov 2018 – March 2023 
  2. Principal Investigator in the project financed by Centre of Technology Transfer Jagiellonian University as a part of competition for pre-implementation studies “Application of Raman spectroscopy method to diagnose kidney disease by analysis of urinary microvesicles”, March 2020 – March 2021. 
  3. Contractor in the project financed by Centre of Technology Transfer Jagiellonian University (Incubator Plus project), Oct 2017 – Sept 2018 
  4. Principal Investigator: „Molecular and spectroscopic analysis of urinary extracellular vesicles from diabetic patients” (DSC project for young researches), May 2016 – Mar 2017 
  5. Principal Investigator: „The role of adhesion molecules and the surface charge in interactions between microvesicles and cell” (DSC project for young researchers), May 2015 – Mar 2016 
  6. Contractor in the project: „The role of microparticles in pathomechanisms of diabetic retinopathy – analysis of intercellular communication mechanisms in endothelial aging”, National Science Center, OPUS project ID: 2012/07/B/NZ5/02510, Oct 2014 – June 2017 
  7. Contractor in the project: ”Vascular endothelium in life-style diseases: from basic research to the offer of an innovative endothelium-targeted therapeutic”, Grant No. POIG.01.01.02-00-069/09, Nov 2013 – Sept 2014. 

Publications

  1. Kamińska A, Roman M, Wróbel A, et al. Raman spectroscopy of urinary extracellular vesicles to stratify patients with chronic kidney disease in type 2 diabetes. Nanomedicine, vol. 39, 2022. 
  2. Stępień E, Kamińska A, Surman M, et al. Fourier-Transform InfraRed (FT-IR) spectroscopy to show alterations in molecular composition of EV subpopulations from melanoma cell lines in different malignancy. Biochemistry and Biophysics Reports, vol. 25, 2021. 
  3. Kamińska A, Marzec ME, Stępień E. Design and optimization of a biosensor surface functionalization to effectively capture urinary extracellular vesicles. Molecules, vol. 26 no. 16, 2021 
  4. Drożdż A, Kamińska A, Surman M, et al. Low-vacuum filtration as an alternative extracellular vesicles concentration method: a comparison with ultracentrifugation and differential centrifugation. Pharmaceutics, vol. 12 no. 9, 2020 
  5. Kamińska A, Gajos K, Woźnicka O, et al. Using a lactadherin-immobilized silicon surface for capturing and monitoring plasma microvesicles as a foundation for diagnostic device development. Anal Bioanal Chemistry, 2020 vol. 412 no. 29, p. 8093-8106. 
  6. Roman M, Kamińska A, Drożdż A, et al. Raman spectra signatures of urinary extracellular vesicles from diabetic patients and hyperglycemic endothelial cells as potential biomarkers in diabetes. Nanomedicine, 2019 vol. 17, p. 137-149. 
  7. Stępień EŁ, Durak-Kozica M, Kamińska A, Targosz-Korecka M, Libera M, Tylko G, et al. Circulating ectosomes: Determination of angiogenic microRNAs in type 2 diabetes. Theranostics 2018,  
  8. Kamińska A, Enguita FJ, Stępień EŁ. Lactadherin: An unappreciated haemostasis regulator and potential therapeutic agent. Vascul Pharmacol 2018;101:21-28 
  9. Kamińska A, Platt M, Kasprzyk J, et al. Urinary extracellular vesicles: potential biomarkers of renal failure in diabetic patients. J Diabetes Res 2016: 5741518 
  10. Gajos K, Kamińska A, Awsiuk K, et al. Immobilization and detection of platelet-derived microvesicles on silicon substrate: cytometric and spectroscopic approach. Anal Bioanal Chem 2017, 409(4):1109-1119 

Patents

  1. Methods of determining the degree of kidney damage occurring in patients with diabetes, patent grate date: 2021-10-25, patent no. PAT.240124 
  2. A method of detection of the kidney damage in patients with diabetes, patent grade date: 2021-01-25, patent no. PAT.237810 
  3. A method of detecting and diagnosing the progression of diabetes, patent grade date: 2020-05-25, patent no. PAT.235682 

Awards

Winner of the Polish Intelligent Development Award 2020 under the patronage of the President of the Patent Office RP, dr Alicji Adamczak in the category: Scientist of the future for the implementation of the project entitled: “Design and construction of the device for the capture of extracellular microvesicles and the assessment of their molecular composition for application in the diagnostics of diabetes complications

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